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FABP12 Rabbit pAb

FABP12 Rabbit pAb

     
  • 14 - FABP12 Rabbit pAb AP55051
    Paraformaldehyde-fixed, paraffin embedded (Human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FABP12) Polyclonal Antibody, Unconjugated (AP55051) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF
Primary Accession A6NFH5
Reactivity Human, Mouse
Predicted Rat, Dog, Horse, Sheep
Host Rabbit
Clonality Polyclonal
Calculated MW 15565 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human FABP12
Epitope Specificity 21-120/140
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions Fatty acid-binding proteins, designated FABPs, are a family of homologous cytoplasmic proteins that are expressed in a highly tissue-specific manner and play an integral role in the balance between lipid and carbohydrate metabolism. FABPs mediate fatty acid (FA) and/or hydrophobic ligand uptake, transport and targeting within their respective tissues. The mechanisms underlying these actions can give rise to both passive diffusional uptake and protein-mediated transmembrane transport of FAs. FABP12 (fatty acid-binding protein 12) is a 132 amino acid protein that belongs to the calycin superfamily and fatty-acid binding protein family. Highly expressed in adult retina and testis, FABP12 may function in lipid transport. The gene encoding FABP12 maps to mouse chromosome 3 A1.
Additional Information
Gene ID 646486
Other Names Fatty acid-binding protein 12, FABP12
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name FABP12
Function May play a role in lipid transport.
Tissue Location Expressed in a number of retinoblastoma cell lines.
Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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