GABA Transporter 3 Rabbit pAb

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (AP55109)
Dilution: 1 µg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1 µg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Sample:A431 (human)cell Lysate at 40 ug
Primary: Anti-GABA Transporter 3 (AP55109)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71 kD
Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-GABA Transporter 3 antibody (AP55109)
Dilution: 1 µg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1 µg /test.
Protocol
The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P48066
Reactivity	Human
Predicted	Rat, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	70606 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GABA Transporter 3/GAT 3
Epitope Specificity	531-632/632
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Membrane; Multi-pass membrane protein.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Gamma-aminobutyric acid (GABA) is a major inhibitory neurotransmitter. GABAergic neurotransmission is terminated by the uptake of GABA into the presynaptic terminal and the surrounding astroglial cells by sodium-dependent transporters, such as SLC6A11 (Borden et al., 1994 [PubMed 7874447]).[supplied by OMIM, Nov 2010]

Additional Information
Gene ID	6538
Other Names	Sodium- and chloride-dependent GABA transporter 3, GAT-3, Solute carrier family 6 member 11, SLC6A11, GABT3, GAT3
Dilution	WB=1:500-2000,Flow-Cyt=1ug/test
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SLC6A11
Synonyms	GABT3, GAT3
Function	Mediates sodium- and chloride-dependent transport of gamma- aminobutyric acid (GABA) (PubMed:7874447). Can also mediate transport of beta-alanine and to a lower extent that of taurine and hypotaurine (By similarity).
Cellular Location	Cell membrane {ECO:0000250\|UniProtKB:P31647}; Multi-pass membrane protein
Tissue Location	Widespread distribution in the brain.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.