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GABPA Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GABPA) Polyclonal Antibody, Unconjugated (AP55110) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- GABPA (AP55110)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q06546
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	51295 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GABPA
Epitope Specificity	51-150/454
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus.
SIMILARITY	Belongs to the ETS family. Contains 1 ETS DNA-binding domain. Contains 1 PNT (pointed) domain.
SUBUNIT	Heterotetramer of two alpha and two beta subunits.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The transcription factor GA-binding protein (GABP) is composed of two subunits, the Ets-related GABP-alpha and a GABP-alpha-associated subunit, GABP beta. GABP alpha binds to a specific DNA sequence and GABP beta exists as b1 and b2 splice variants that differ in their C-termini. In primary neuronal cultures, GABP beta is expressed in both the cytoplasm and the nucleus, whereas GABP alpha is expressed mainly in the nucleus. GABP is constitutively expressed as either a GABP alpha beta heterodimer or a GABP alpha b heterotetramer, both of which can modify GABP-dependent transcription in vitro and in vivo. The GABP alpha beta tetrameric complex performs many different functions, such as stimulating transcription of the adenovirus E4 gene, differentially activating BRCA1 expression in human breast cell lines, potentiating Tat-mediated activation of long terminal repeat promoter transcription and viral replication in certain cell types, acting as a coordinator of mitochrondrial and nuclear transcription for cytochrome oxidase in neurons and assisting in the regulation of rpL32 gene transcription.

Additional Information
Gene ID	2551
Other Names	GA-binding protein alpha chain, GABP subunit alpha, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60, GABPA, E4TF1A
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	GABPA
Synonyms	E4TF1A
Function	Transcription factor capable of interacting with purine rich repeats (GA repeats). Positively regulates transcription of transcriptional repressor RHIT/ZNF205 (PubMed:22306510).
Cellular Location	Nucleus.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol