GLT1D1 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (human colon cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GLT1D1) Polyclonal Antibody, Unconjugated (AP55151) at 1:5000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q96MS3
Reactivity	Rat, Human
Predicted	Pig, Dog, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	38507 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GLT1D1
Epitope Specificity	251-346/346
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted (Potential).
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	GLT1D1 (Glycosyltransferase 1 Domain Containing 1) is a Protein Coding gene. GO annotations related to this gene include transferase activity, transferring glycosyl groups.

Additional Information
Gene ID	144423
Other Names	Glycosyltransferase 1 domain-containing protein 1, 2.4.-.-, GLT1D1
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.