> 首页 > 产品 > 一抗 > 其他 > COLGALT1 Rabbit pAb

COLGALT1 Rabbit pAb

Sample:
Lane 1: Mouse Placenta tissue lysates
Lane 2: Mouse Liver tissue lysates
Lane 3: Rat Placenta tissue lysates
Lane 4: Rat Spleen tissue lysates
Lane 5: Rat Liver tissue lysates
Lane 6: Human THP-1 cell lysates
Primary: Anti-COLGALT1 (AP55152) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 68 kDa
Blank control:Mouse spleen.
Primary Antibody (green line): Rabbit Anti-GLT25D1 antibody (AP55152)
Dilution: 2 µg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1 µg /test.
Protocol
The cells were fixed with 70% ethanol (10min at room temperature) and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

产品详情
实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q8NBJ5
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	71636 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GLT25D1
Epitope Specificity	531-622/622
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Endoplasmic reticulum lumen. Note: Colocalized with PLOD3 and mannose binding lectin.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	GLT25D1 is a 622 amino acid protein that localizes to the lumen of the endoplasmic reticulum. Ubiquitously expressed with higher levels in placenta, heart, lung and spleen, GLT25D1 is a member of the glycosyltransferase 25 family. The beta-galactosyltransferase activity of GLT25D1 allows the transfer of beta-galactose to hydroxylysine residues of collagen. GLT25D1 is considered an important target for investigating the biological significance of collagen glycosylation and the importance of posttranslational modification in the etiology of connective tissue disorders. The gene encoding GLT25D1 is located on human chromosome 19, which consists of over 63 million bases, houses approximately 1,400 genes and is recognized for having the greatest gene density of the human chromosomes.

Additional Information
Gene ID	79709
Other Names	Procollagen galactosyltransferase 1, 2.4.1.50, Collagen beta(1-O)galactosyltransferase 1, ColGalT 1, Glycosyltransferase 25 family member 1, Hydroxylysine galactosyltransferase 1, COLGALT1, GLT25D1
Dilution	WB=1:500-2000,ELISA=1:5000-10000,ICC/IF=1:50-200
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	COLGALT1
Synonyms	GLT25D1
Function	Beta-galactosyltransferase that transfers beta-galactose to hydroxylysine residues of type I collagen (PubMed:19075007, PubMed:22216269, PubMed:27402836). By acting on collagen glycosylation, facilitates the formation of collagen triple helix (PubMed:27402836). Also involved in the biosynthesis of collagen type IV (PubMed:30412317).
Cellular Location	Endoplasmic reticulum lumen {ECO:0000255\|PROSITE- ProRule:PRU10138, ECO:0000269\|PubMed:20470363}. Note=Colocalized with PLOD3 and mannose binding lectin/MBL2.
Tissue Location	Ubiquitous with higher levels in placenta, heart, lung and spleen.