> 首页 > 产品 > 一抗 > 其他 > GLUT6/GLUT9 Polyclonal Antibody

GLUT6/GLUT9 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated(AP55157) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GLUT6 Polyclonal Antibody, Unconjugated(AP55157) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Brain (Mouse) Lysate at 40 ug
Primary: Anti- GLUT6'GLUT9 (AP55157)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- GLUT6'GLUT9 (AP55157)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q9UGQ3
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	54539 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GLUT6
Epitope Specificity	201-300/507
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cell membrane. The dileucine internalization motif is critical for intracellular sequestration.
SIMILARITY	Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The oxidation of glucose functions as the dominant source of metabolic energy for mammals. The plasma membrane is impermeable to glucose, so the cellular uptake of this important nutrient is achieved by facultative hexose transproters (Gluts). Gluts are integral membrane proteins that transport glucose and related hexoses. Glucose binds to a Glut on one side of the membrane which provokes a conformational change causing it to release glucose to the other side. Members of the Glut family may enhance the metabolic activity of tumor cells. Glut6 is part of the third out of three classes of Gluts. Glut6 is mainly expressed in the brain, spleen and peripheral leukocytes. It appears to be regulated by subcellular redistribution, because it is targeted to intracellular compartments by di-leucine motifs, recycling itself in a Dynamin-dependent manner.

Additional Information
Gene ID	11182
Other Names	Solute carrier family 2, facilitated glucose transporter member 6, Glucose transporter type 6, GLUT-6, SLC2A6 (HGNC:11011)
Target/Specificity	Highly expressed in brain, spleen and peripheral blood leukocytes.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	SLC2A6 (HGNC:11011)
Function	Probable sugar transporter that acts as a regulator of glycolysis in macrophages (Probable). Does not transport glucose (PubMed:30431159).
Cellular Location	Lysosome membrane; Multi-pass membrane protein
Tissue Location	Highly expressed in brain, spleen and peripheral blood leukocytes.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol