GPX2 Rabbit pAb

Sample:
Lane 1: Mouse Liver tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-GPX2 (AP55158) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (GPX2) Polyclonal Antibody, Unconjugated (AP55158) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	P18283
Reactivity	Rat, Mouse
Predicted	Dog, Human, Rabbit, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	21954 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GPX2/Glutathione Peroxidase 2
Epitope Specificity	41-140/190
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm. Mainly cytoplasmic.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1–8). GPx-1 plays an important role in the antioxidant defense of the vascular wall and neural cells in response to oxidative stress. GPx-2 is the major isoform in the lungs and its basal or inducible expression is dependent on Nrf2. GPx-3 is under regulation by hypoxic stress and the expression and deficiency of GPx-3 is associated with cardiovascular disease and stroke. GPx-5 is selenium-independent; it is bound to the acrosome of sperm, where it may protect sperm from premature acrosome reaction in the epididymis.

Additional Information
Gene ID	2877
Other Names	Glutathione peroxidase 2, GPx-2, GSHPx-2, 1.11.1.9, Gastrointestinal glutathione peroxidase, Glutathione peroxidase-gastrointestinal, GPx-GI, GSHPx-GI, Glutathione peroxidase-related protein 2, GPRP-2, Phospholipid hydroperoxide glutathione peroxidase GPX2, 1.11.1.12, GPX2 {ECO:0000303\|Ref.9, ECO:0000312\|HGNC:HGNC:4554}
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	GPX2 {ECO:0000303\|Ref.9, ECO:0000312\|HGNC:HGNC:4554}
Function	Catalyzes the reduction of hydroperoxides in a glutathione- dependent manner thus regulating cellular redox homeostasis (PubMed:36608588, PubMed:8428933). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed:36608588, PubMed:8428933). Cannot reduce phosphatidycholine hydroperoxide (PubMed:8428933).
Cellular Location	Cytoplasm, cytosol.
Tissue Location	Mostly in liver and gastrointestinal tract, not found in heart or kidney.