癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
GOSR1 Rabbit pAb
GOSR1 Rabbit pAb
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | O95249 |
| Reactivity | Mouse, Rat |
| Predicted | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 28613 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human GOSR1/GS28 |
| Epitope Specificity | 151-250/250 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cell Membrane, Endoplasmic reticulum and Golgi Apparatus |
| SIMILARITY | Belongs to the GOSR1 family. |
| SUBUNIT | Component of several multiprotein Golgi SNARE complexes. Identified in a SNARE complex with BET1, STX5 and YKT6, in a SNARE complex with BET1L, STX5 and YKT6, in a SNARE complex with STX5, GOSR2, SEC22B and BET1, and in complex with STX5 and COG3. Interacts with GABARAPL2 (By similarity). |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum and delivers them after covalent modification to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF) to form the highly stable SNAP receptor (SNARE) complex. The formation of a SNARE complex pulls the vesicle and target membranes together and may provide the energy to drive the fusion of the lipid bilayers. Golgi SNARE 27 kDa (GS27) and GS28 belong to the SNARE protein family and are important trafficking proteins between the endoplasmic reticulum and the Golgi and between Golgi subcompartments. GS27 and GS28 both exist as cytoplasmically oriented integral membrane proteins. The human GS27 gene, which maps to chromosome 17q21, is located near a locus implicated in familial essential hypertension, indicating that it is a potential candidate gene for this disease. The human GS28 gene maps to chromosome 17q11. |
| Gene ID | 9527 |
|---|---|
| Other Names | Golgi SNAP receptor complex member 1, 28 kDa Golgi SNARE protein, 28 kDa cis-Golgi SNARE p28, GOS-28, GOSR1, GS28 |
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | GOSR1 |
|---|---|
| Synonyms | GS28 |
| Function | Involved in transport from the ER to the Golgi apparatus as well as in intra-Golgi transport. It belongs to a super-family of proteins called t-SNAREs or soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein receptor. May play a protective role against hydrogen peroxide induced cytotoxicity under glutathione depleted conditions in neuronal cells by regulating the intracellular ROS levels via inhibition of p38 MAPK (MAPK11, MAPK12, MAPK13 and MAPK14). Participates in docking and fusion stage of ER to cis-Golgi transport. Plays an important physiological role in VLDL-transport vesicle-Golgi fusion and thus in VLDL delivery to the hepatic cis-Golgi. |
| Cellular Location | Golgi apparatus membrane; Single-pass type IV membrane protein. Note=Localizes throughout the Golgi apparatus, with lowest levels in the trans-Golgi network (By similarity). Enriched on vesicular components at the terminal rims of the Golgi. Found in Golgi microtubules at low temperature (15 degrees Celsius). |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
In eukaryotic cells, the Golgi apparatus receives newly synthesized proteins from the endoplasmic reticulum and delivers them after covalent modification to their destination in the cell. For membrane-directed proteins this process is believed to be carried out via vesicular transport. Correct vesicular transport is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF) to form the highly stable SNAP receptor (SNARE) complex. The formation of a SNARE complex pulls the vesicle and target membranes together and may provide the energy to drive the fusion of the lipid bilayers. Golgi SNARE 27 kDa (GS27) and GS28 belong to the SNARE protein family and are important trafficking proteins between the endoplasmic reticulum and the Golgi and between Golgi subcompartments. GS27 and GS28 both exist as cytoplasmically oriented integral membrane proteins. The human GS27 gene, which maps to chromosome 17q21, is located near a locus implicated in familial essential hypertension, indicating that it is a potential candidate gene for this disease. The human GS28 gene maps to chromosome 17q11.
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