RUSC1 Rabbit pAb

Sample:Hela (Human)Cell Lysate at 40 ug
Primary: Anti-RUSC1(AP55238)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 96kD
Observed band size: 105kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RUSC1) Polyclonal Antibody, Unconjugated (AP55238) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9BVN2
Reactivity	Human, Mouse
Predicted	Rat, Pig, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	96444 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human RUSC1
Epitope Specificity	281-380/902
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm. Nucleus. Note=Translocated to the nuclear envelope upon stimulation with NGF.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	RUSC1 is a 902 amino acid protein that contains a RUN domain and a SH3 domain. RUSC1’s RUN domain is necessary for NGF induced nuclear redistribution. RUSC1 is a putative signaling adapter which may play a role in neuronal differentiation. RUSC1 seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK. RUSC2 (RUN and SH3 domain containing 2), also known as Iporin, is a 1,516 amino acid cytoplasmic protein that is widely expressed, with highest levels in brain and testis. The RUN domain of RUSC2 is required for interaction with Rab 1A, Rab 1B and GM130. It is thought that RUSC2 may possibly function as a connector between endoplasmic reticulum (ER) derived vesicle targets triggered by the Rab 1 GTPases and a signaling pathway regulated by molecules containing SH3 and/or poly-proline regions. RUSC2 also consists of a SH3 domain, suggesting a role in protein-protein interactions.

Additional Information
Gene ID	23623
Other Names	AP-4 complex accessory subunit RUSC1, New molecule containing SH3 at the carboxy-terminus, Nesca, RUN and SH3 domain-containing protein 1, RUSC1 {ECO:0000303\|PubMed:30262884, ECO:0000312\|HGNC:HGNC:17153}
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RUSC1 {ECO:0000303\|PubMed:30262884, ECO:0000312\|HGNC:HGNC:17153}
Function	Associates with the adapter-like complex 4 (AP-4) and may therefore play a role in vesicular trafficking of proteins at the trans-Golgi network (PubMed:30262884). Signaling adapter which plays a role in neuronal differentiation (PubMed:15024033). Involved in regulation of NGF-dependent neurite outgrowth (PubMed:15024033). May play a role in neuronal vesicular trafficking, specifically involving pre-synaptic membrane proteins (By similarity). Seems to be involved in signaling pathways that are regulated by the prolonged activation of MAPK (PubMed:15024033). Can regulate the polyubiquitination of IKBKG and thus may be involved in regulation of the NF-kappa-B pathway (PubMed:19365808).
Cellular Location	Cytoplasm. Nucleus. Cytoplasm, cytoskeleton {ECO:0000250\|UniProtKB:Q8BG26}. Cytoplasmic vesicle {ECO:0000250\|UniProtKB:Q8BG26}. Early endosome {ECO:0000250\|UniProtKB:Q8BG26}. Postsynaptic density {ECO:0000250\|UniProtKB:Q8BG26}. Golgi apparatus {ECO:0000250\|UniProtKB:Q8BG26}. Note=Translocated to the nuclear envelope upon stimulation with NGF (PubMed:15024033). Associated with membranes and microtubules (By similarity) {ECO:0000250\|UniProtKB:Q8BG26, ECO:0000269\|PubMed:15024033}
Tissue Location	Predominantly expressed in brain.