EXD2 Rabbit pAb

Sample: HepG2 (Human)Cell Lysate at 40 ug
Primary: Anti-EXD2(AP55658)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 70kD
Observed band size: 56kD
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXD2) Polyclonal Antibody, Unconjugated (AP55658) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9NVH0
Reactivity	Rat, Human, Mouse
Predicted	Pig, Dog, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	70353 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human EXD2
Epitope Specificity	351-621/621
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Gene ID	55218
Other Names	Exonuclease 3'-5' domain-containing protein 2, 3.1.11.1, 3'-5' exoribonuclease EXD2, 3.1.13.-, Exonuclease 3'-5' domain-like-containing protein 2, EXD2 {ECO:0000303\|PubMed:26807646, ECO:0000312\|HGNC:HGNC:20217}
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	EXD2 {ECO:0000303\|PubMed:26807646, ECO:0000312\|HGNC:HGNC:20217}
Function	Exonuclease that has both 3'-5' exoribonuclease and exodeoxyribonuclease activities, depending on the divalent metal cation used as cofactor (PubMed:29335528, PubMed:31127291). In presence of Mg(2+), only shows 3'-5' exoribonuclease activity, while it shows both exoribonuclease and exodeoxyribonuclease activities in presence of Mn(2+) (PubMed:29335528, PubMed:31127291). Acts as an exoribonuclease in mitochondrion, possibly by regulating ATP production and mitochondrial translation (PubMed:29335528). Also involved in the response to DNA damage (PubMed:26807646, PubMed:31255466). Acts as 3'- 5' exodeoxyribonuclease for double-strand breaks resection and efficient homologous recombination (PubMed:20603073, PubMed:26807646). Plays a key role in controlling the initial steps of chromosomal break repair, it is recruited to chromatin in a damage-dependent manner and functionally interacts with the MRN complex to accelerate resection through its 3'-5' exonuclease activity, which efficiently processes double-stranded DNA substrates containing nicks (PubMed:26807646). Also involved in response to replicative stress: recruited to stalled forks and is required to stabilize and restart stalled replication forks by restraining excessive fork regression, thereby suppressing their degradation (PubMed:31255466).
Cellular Location	Mitochondrion outer membrane; Single-pass membrane protein {ECO:0000255, ECO:0000269\|PubMed:31127291} Mitochondrion matrix. Nucleus. Chromosome. Note=Mainly localizes to the mitochondrial outer membrane (PubMed:29599527, PubMed:31127291). May translocate to the nucleus in response to DNA damage; however mechanism that explain nuclear localization are unknown and require experimental evidences (PubMed:26807646). Recruited to replication forks following replication stress (PubMed:31255466).