EXOC6B Rabbit pAb

Sample:Hela (Human)Cell Lysate at 40 ug
Primary: Anti-EXOC6B(AP55663)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 94kD
Paraformaldehyde-fixed, paraffin embedded (Rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC6B) Polyclonal Antibody, Unconjugated (AP55663) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9Y2D4
Reactivity	Human, Rat
Predicted	Mouse, Dog, Pig, Horse, Rabbit, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	94201 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human EXOC6B
Epitope Specificity	721-811/811
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Belongs to the SEC15 family.
SUBUNIT	The exocyst complex is composed of SEC3, SEC5, SEC6, SEC8, SEC10, SEC15, EXO70 and EXO84.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	In yeast and rat, Sec15 is part of a multiprotein complex that is required for targeted exocytosis.[supplied by OMIM, Apr 2004]

Additional Information
Gene ID	23233
Other Names	Exocyst complex component 6B, Exocyst complex component Sec15B, SEC15-like protein 2, EXOC6B, KIAA0919, SEC15B, SEC15L2
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	EXOC6B
Synonyms	KIAA0919, SEC15B, SEC15L2
Function	Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.