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CD361 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Tissue/cell: mosue embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(AP55672) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: Human lung cancer tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(AP55672) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CD361 Polyclonal Antibody, Unconjugated(AP55672) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- CD361 (AP55672) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 66 kD

产品详情
实验流程

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	P34910
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	48666 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human CD361
Epitope Specificity	151-250/448
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Membrane.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	EVI2B is a 448 amino acid protein which functions in the differentiation of melanocytes and keratinocytes. Lying within an intron of the Neurofibromin gene, the gene encoding EVI2B is transcribed from the telomere toward the centromere, which is opposite the transcription direction of the Neurofibromin gene. EVI2B is a single-pass transmembrane protein containing an extracellular domain with 4 glycosylation sites, a N-terminal signal peptide, a cytoplasmic hydrophilic domain and a hydrophobic transmembrane domain. Due to evidence suggesting that gene encoding the mouse homolog lies within a viral integration site that has been identitified in retrovirus-induced myeloid tumors, the gene encoding EVI2B may function as an oncogene in these tumor types. With expression in peripheral blood mononuclear cells, fibroblasts, bone marrow and EBV-transformed lymphoblastoid cell lines, EVI2B is implicated in leukemogenesis.

Additional Information
Gene ID	2124
Other Names	Protein EVI2B, Ecotropic viral integration site 2B protein homolog, EVI-2B, CD361, EVI2B {ECO:0000303\|PubMed:1903357, ECO:0000312\|HGNC:HGNC:3500}
Target/Specificity	Bone marrow, peripheral blood mononuclear cells, fibroblasts and Epstein-Barr virus-transformed lymphoblastoid cell lines.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	EVI2B {ECO:0000303\|PubMed:1903357, ECO:0000312\|HGNC:HGNC:3500}
Function	Required for granulocyte differentiation and functionality of hematopoietic progenitor cells through the control of cell cycle progression and survival of hematopoietic progenitor cells.
Cellular Location	Membrane; Single-pass type I membrane protein.
Tissue Location	Bone marrow, peripheral blood mononuclear cells, fibroblasts and Epstein-Barr virus-transformed lymphoblastoid cell lines. Strongly expressed in granulocytic cells, and weakly on lymphocytes cells.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol