HEV Capsid protein Rabbit pAb
HEV Capsid protein Rabbit pAb
- 产品详情
- 实验流程
- 背景知识
Application
| WB, IHC-P, IHC-F, IF |
|---|---|
| Predicted | Hepatitis E Virus |
| Host | Rabbit |
| Clonality | Polyclonal |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from Hepatitis E Virus Capsid protein |
| Epitope Specificity | 301-400/660 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Virion (Probable). Host cytoplasm. Host cell surface. Note=Initially cotranslationally translocated into the ER from where it is retrotranslocated to the cytoplasm. A fraction is also observed on the cell surface. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | The Hepatitis E virus is the causative agent of Hepatitis E. Its taxonomic name is Orthohepevirus A. The viral genome a single-strand of positive-sense RNA that is approximately 7200 bases in length. It encodes 3 proteins (O1, O2, O3), two of which are polyproteins, that is, they are cleaved into fragments which carry out the actual functions of the virus. The O1 protein consists of 7 such fragments, namely Met (Methyltransferase), Y (Y-domain), Plp (Papain like protease), V (proline-rich variable region), X (X-domain, macro-domain), Hel (Helicase), and Rdrp (RNA dependent RNA polymerase). The Pvx domain is a fusion protein consisting of the Plp, V and X domains. The O3 protein is encoded by a single open reading frame (ORF3). The O2 protein encodes the capsid, which is composed of 3 domains, namely the shell domain (S) and two protruding domains (P1, P2). Numbers in the figure indicate positions in the RNA sequence. |
| Other Names | Capsid protein, Protein ORF2, pORF2, ORF2 |
|---|---|
| Dilution | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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