癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
ZNFX1 Rabbit pAb
ZNFX1 Rabbit pAb
- 产品详情
- 实验流程
- 背景知识
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9P2E3 |
| Predicted | Rat, Human, Mouse, Horse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 220227 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human ZNFX1 |
| Epitope Specificity | 1601-1700/1918 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | ZNFX1 is a 1918 amino acid nuclear protein that is widely expressed and contains six NF-X1-type zinc fingers, which are presumed to function as zinc binding domains. The gene encoding ZNFX1 maps to human chromosome 20, which contains nearly 63 million bases that encode over 600 genes, some of which are associated with Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, spinal muscular atrophy, ring chromosome 20 epilepsy syndrome and Alagille syndrome. There are two isoforms of ZNFX1 that are produced as a result of alternative splicing events. |
| Gene ID | 57169 |
|---|---|
| Other Names | NFX1-type zinc finger-containing protein 1, ZNFX1 {ECO:0000303|PubMed:33872655, ECO:0000312|HGNC:HGNC:29271} |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | ZNFX1 {ECO:0000303|PubMed:33872655, ECO:0000312|HGNC:HGNC:29271} |
|---|---|
| Function | RNA-activated non-canonical E3 ubiquitin ligase required for cell survival during interferon (IFN) responses. Functions as an ATP- dependent 5'-> 3' RNA translocase that selectively recognizes long single-stranded RNA (ssRNA) molecules (>100 nt) from viral or host origin during immune activation. Upon ssRNA binding, oligomerizes to assemble a split E3 active site in trans, involving the Z-RING domain that binds and activates E2-ubiquitin conjugates, such as UBE2D2, and the RZ catalytic domain that mediates ubiquitin transfer. Catalyzes hydroxyl ubiquitination of the 2'-OH of ssRNA ribose and auto- ubiquitination, generating mainly 'Lys-48'- and 'Lys-6'-linked polyubiquitin chains (PubMed:40876457). Forms ubiquitin-coated RNA condensates and stress granules, thereby regulating RNA metabolism, limiting excessive immune signaling, and preventing IFN-induced cell death (PubMed:33872655, PubMed:40876457). Also required for immunity against some bacteria, such as mycobacteria (PubMed:33876776). Inhibits NLRP3 inflammasome activation by sequestering NLRP3 in the cytoplasm and preventing its translocation to trans-Golgi network vesicles during the resting state. Upon inflammasome activation, is cleaved by CASP1, establishing a feed-forward loop that amplifies NLRP3 inflammasome activity (PubMed:39333773). |
| Cellular Location | Mitochondrion outer membrane {ECO:0000250|UniProtKB:Q8R151}. Cytoplasm, Stress granule. Cytoplasm, cytosol |
| Tissue Location | Widely expressed. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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