IRAK1BP1 Rabbit pAb

Protein: MCF-7(human) lyates at 40ug;
Primary: Rabbit Anti-IRAK1BP1 (AP56361) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:29 kD
Observed band size:33 kD
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IRAK1BP1 Polyclonal Antibody, Unconjugated(AP56361) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q5VVH5
Reactivity	Human, Mouse
Predicted	Rat, Rabbit
Host	Rabbit
Clonality	Polyclonal
Calculated MW	29106 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human IRAK1BP1
Epitope Specificity	51-150/260
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasmic and Nuclear
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	IRAK1BP1 is a component of the IRAK1 dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. It acts by enhancing RELA transcriptional activity.

Additional Information
Gene ID	134728
Other Names	Interleukin-1 receptor-associated kinase 1-binding protein 1, IRAK1-binding protein 1, IRAK1BP1
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	IRAK1BP1
Function	Component of the IRAK1-dependent TNFRSF1A signaling pathway that leads to NF-kappa-B activation and is required for cell survival. Acts by enhancing RELA transcriptional activity (By similarity).
Cellular Location	Cytoplasm. Nucleus.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.