DELE Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q14154
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	55920 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human DELE
Epitope Specificity	401-500/515
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Mitochondrial
SIMILARITY	Contains 7 TPR repeats.
SUBUNIT	Interacts with DAP3.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	KIAA0141 is detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon. It contains seven TPR repeats. Its function is unknown.

Additional Information
Gene ID	9812
Other Names	DAP3-binding cell death enhancer 1, DAP3-binding cell death enhancer 1, long form, DELE1(L), Death ligand signal enhancer, DAP3-binding cell death enhancer 1 short form, DELE1(S), S-DELE1, DELE1 (HGNC:28969)
Target/Specificity	Detected in liver, skeletal muscle, kidney,pancreas, spleen, thyroid, testis, ovary, small intestine and colon.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	DELE1 {ECO:0000303\|PubMed:32132706, ECO:0000312\|HGNC:HGNC:28969}
Function	Protein kinase activator that acts as a key activator of the integrated stress response (ISR) following various stresses, such as iron deficiency, mitochondrial stress or mitochondrial DNA breaks (PubMed:32132706, PubMed:32132707, PubMed:35388015, PubMed:37327776, PubMed:37550454, PubMed:37832546, PubMed:38340717). Detects impaired protein import and processing in mitochondria, activating the ISR (PubMed:35388015). May also required for the induction of death receptor-mediated apoptosis through the regulation of caspase activation (PubMed:20563667).
Cellular Location	[DAP3-binding cell death enhancer 1]: Mitochondrion. Mitochondrion outer membrane. Mitochondrion inner membrane. Note=Imported in the mitochondrial matrix in absence of stress, leading to its degradation by LONP1 (PubMed:37327776). Localizes at the mitochondrial surface in response to iron deficiency: iron deficiency impairs mitochondrial import, promoting localization at the mitochondrial surface and stabilization (PubMed:37327776). Associates with the mitochondrion inner membrane in response to mitochondrial stress, leading to its proteolytic processing by OMA1, and generation of the AP3-binding cell death enhancer 1 short form (DELE1(S) or S-DELE1) (PubMed:32132707)
Tissue Location	Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon