TRIML2 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TRIML2) Polyclonal Antibody, Unconjugated (AP56567) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q8N7C3
Reactivity	Mouse
Predicted	Rat, Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	49893 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human TRIML2
Epitope Specificity	221-320/387
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The specific function of TRIML2 is not yet known.

Additional Information
Gene ID	205860
Other Names	Probable E3 ubiquitin-protein ligase TRIML2, 2.3.2.27, RING-type E3 ubiquitin transferase TRIML2, SPRY domain-containing protein 6, Tripartite motif family-like protein 2, TRIML2 (HGNC:26378), SPRYD6
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.