SFRS2IP Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (rat lymphoid tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRS2IP) Polyclonal Antibody, Unconjugated (AP56651) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q99590
Reactivity	Rat, Mouse
Predicted	Pig, Human, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	164652 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SFRS2IP
Epitope Specificity	301-400/1463
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nuclear
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	SFRS2IP plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.

Additional Information
Gene ID	9169
Other Names	Protein SCAF11, CTD-associated SR protein 11, Renal carcinoma antigen NY-REN-40, SC35-interacting protein 1, SR-related and CTD-associated factor 11, SRSF2-interacting protein, Serine/arginine-rich splicing factor 2-interacting protein, Splicing factor, arginine/serine-rich 2-interacting protein, Splicing regulatory protein 129, SRrp129, SCAF11, CASP11, SFRS2IP, SIP1, SRSF2IP
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SCAF11
Synonyms	CASP11, SFRS2IP, SIP1, SRSF2IP
Function	Plays a role in pre-mRNA alternative splicing by regulating spliceosome assembly.
Cellular Location	Nucleus.
Tissue Location	Widely expressed..

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.