OSBPL6 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSBPL6) Polyclonal Antibody, Unconjugated (AP56729) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSBPL6) Polyclonal Antibody, Unconjugated (AP56729) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-OSBPL6 (AP56729) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 106 kD
Observed band size: 106 kD

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC
Primary Accession	Q9BZF3
Reactivity	Rat, Pig, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	106306 Da

Additional Information
Gene ID	114880
Other Names	Oxysterol-binding protein-related protein 6, ORP-6, OSBP-related protein 6, OSBPL6, ORP6
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	OSBPL6 (HGNC:16388)
Synonyms	ORP6
Function	Regulates cellular transport and efflux of cholesterol (PubMed:26941018). Plays a role in phosphatidylinositol-4-phosphate (PI4P) turnover at the neuronal membrane. Localizes at the neurons plasma membrane-endoplasmic reticulum contact sites, and exchanges phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine, PS) from the endoplasmic reticulum for phosphatidylinositol-4-phosphate (1,2- diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate), PI(4)P) from the plasma membrane (By similarity). Binds via its PH domain PI4P, phosphatidylinositol-4,5-diphosphate, phosphatidylinositol-3,4,5- triphosphate, and phosphatidic acid (By similarity). Weakly binds 25- hydroxycholesterol (PubMed:17428193).
Cellular Location	Cytoplasm, cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein. Nucleus envelope. Cell membrane; Peripheral membrane protein. Endosome membrane; Peripheral membrane protein. Note=Co-localizes with OSBPL3 at contact sites between the plasma membrane and the endoplasmic reticulum {ECO:0000250\|UniProtKB:Q8BXR9}
Tissue Location	Expressed in brain and striated muscle (at protein level) (PubMed:14593528). Widely expressed (PubMed:11735225). Expressed in skeletal muscle (PubMed:14593528)