癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SPEF1 Rabbit pAb
SPEF1 Rabbit pAb
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- 背景知识
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9Y4P9 |
| Reactivity | Rat, Mouse |
| Predicted | Pig, Human, Rabbit, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 26987 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human SPEF1 |
| Epitope Specificity | 181-236/236 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Cytoplasm. Cell projection; cilium; flagellum. Cytoplasm; cytoskeleton; cilium axoneme. Note: Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | CLAMP is a 236 amino acid protein that is present in epididymal sperm and localizes to the cell projection, as well as to the cytoplasm. Expressed in lung, brain and testis, CLAMP functions as a microtubule-associated protein that is thought to play a role in microtubule bundling. Human CLAMP exists as two alternatively spliced isoforms and shares a high degree of homology with its mouse counterpart, suggesting a conserved role between species. The gene encoding CLAMP maps to human chromosome 20, which houses over 600 genes and comprises nearly 2% of the human genome. |
| Gene ID | 25876 |
|---|---|
| Other Names | Sperm flagellar protein 1, SPEF1, C20orf28 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SPEF1 |
|---|---|
| Synonyms | C20orf28 |
| Function | Microtubule-associated protein involved in the stabilization of microtubules along the axis of migration during radial intercalation. Promotes the establishment and stabilization of an axis of microtubules required for the active migration of cells into the outer epithelium (By similarity). Microtubule-associated protein that promotes microtubule bundling and stabilizes microtubules against depolymerization in response to cold shock (By similarity). Essential for ciliary central apparatus formation which requires both its microtubule-binding and bundling activities and for ciliary localization of HYDIN and SPAG6 in ependymal cilia (By similarity). Binds actin in intestinal epithelial cells (IECs), essential for IECs survival and contributes to formation of filopodia and lamellipodia in migrating IECs (PubMed:31473225). Regulates planar cell polarity signaling pathway and asymmetric microtubule accumulation in ciliated epithelia (By similarity). |
| Cellular Location | Cytoplasm. Cell projection, cilium, flagellum {ECO:0000250|UniProtKB:Q99JL1} Cytoplasm, cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q0IH24} Apical cell membrane. Basolateral cell membrane. Cytoplasm, cytoskeleton, stress fiber. Cell projection, microvillus. Cell projection, lamellipodium. Cell projection, filopodium. Note=Present in the tails of developing and epididymal sperm, internal to the fibrous sheath and around the outer dense fibers of the sperm flagellum. Also found at the apical tip of cilia (By similarity). Colocalizes with TJP1 and CGN at sites of cell-cell contact in intestinal epithelial cells (PubMed:31473225) {ECO:0000250|UniProtKB:Q0IH24, ECO:0000269|PubMed:31473225} |
| Tissue Location | Expressed in the intestinal epithelial cells (at protein level). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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