SPERT Rabbit pAb

Sample:
Hela(human) Cell Lysate at 40 ug
DU145(human) Cell Lysate at 40 ug
Primary: Anti-SPERT(AP56776)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52kD
Observed band size: 52kD
Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPERT) Polyclonal Antibody, Unconjugated (AP56776) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q8NA61
Reactivity	Rat, Human
Predicted	Mouse, Rabbit, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	51570 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SPERT
Epitope Specificity	1-100/448
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasmic membrane bounded vesicle.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	A variety of morphological and molecular changes are required for spermatozoa formation. These steps are temporally guided by the transcription and translation of several testis-specific genes. SPERT (spermatid associated), also known as CBY2 (chibby homolog 2), spermatid flower-like structure protein or NURIT, is a 448 amino acid novel leucine-zipper protein belonging to the chibby family of proteins.Expressed uniquely in the spermatid flower-like structure, SPERT interacts with Nek1, a member of the NIMA-family kinase family that is associated centrosomal stability and ciliogenesis. Containing a leucine-zipper motif and two coiled-coil regions, SPERT is transcribed through the elongation stage of the spermatids. SPERT is absent from mature spermatozoa and is thought to be involved in transporting proteins that are to be discarded via the residual bodies.

Additional Information
Gene ID	220082
Other Names	Protein chibby homolog 2, Spermatid-associated protein, CBY2 (HGNC:30720), SPERT
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.