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MTFMT Rabbit pAb

MTFMT Rabbit pAb

     
  • 14 - MTFMT Rabbit pAb AP56878
    Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTFMT) Polyclonal Antibody, Unconjugated (AP56878) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF
Primary Accession Q96DP5
Reactivity Mouse
Predicted Rat, Dog, Human, Sheep
Host Rabbit
Clonality Polyclonal
Calculated MW 43832 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human MTFMT
Epitope Specificity 31-130/389
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Mitochondrion.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The protein encoded by this nuclear gene localizes to the mitochondrion, where it catalyzes the formylation of methionyl-tRNA. [provided by RefSeq, Jun 2011]
Additional Information
Gene ID 123263
Other Names Methionyl-tRNA formyltransferase, mitochondrial, MtFMT, 2.1.2.9, MTFMT, FMT, FMT1
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name MTFMT
Synonyms FMT, FMT1
Function Methionyl-tRNA formyltransferase that formylates methionyl- tRNA in mitochondria and is crucial for translation initiation.
Cellular Location Mitochondrion {ECO:0000250|UniProtKB:O77480}.
Research Areas

BACKGROUND

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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