ISX/Intestine specific homeobox Rabbit pAb

Sample:
small intestine (Mouse) Lysate at 40 ug
large intestine (Mouse) Lysate at 40 ug
Primary: Anti-ISX/Intestine specific homeobox (AP56973) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ISX) Polyclonal Antibody, Unconjugated (AP56973) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q2M1V0
Reactivity	Human, Mouse
Predicted	Rat, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	27011 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human ISX/Intestine specific homeobox
Epitope Specificity	81-180/245
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Homeobox genes encode DNA-binding proteins, many of which are thought to be involved in early embryonic development. Homeobox genes encode a DNA-binding domain of 60 to 63 amino acids referred to as the homeodomain. This gene is a member of the RAXLX homeobox gene family. [provided by RefSeq, Jul 2008]

Additional Information
Gene ID	91464
Other Names	Intestine-specific homeobox, RAX-like homeobox, ISX, RAXLX
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ISX
Synonyms	RAXLX
Function	Transcription factor that regulates gene expression in intestine. May participate in vitamin A metabolism most likely by regulating BCO1 expression in the intestine (By similarity).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00108}.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.