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LINS Rabbit pAb

LINS Rabbit pAb

     
  • 1 - LINS Rabbit pAb AP57020
    Sample:
    U251(Human) Cell Lysate at 40 ug
    Primary: Anti-LINS (AP57020) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 86 kD
    Observed band size: 86 kD
  • 14 - LINS Rabbit pAb AP57020
    Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LINS) Polyclonal Antibody, Unconjugated (AP57020) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Primary Accession Q8NG48
Reactivity Human
Host Rabbit
Clonality Polyclonal
Calculated MW 85857 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human LINS
Epitope Specificity 121-220/757
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY Belongs to the protein lines family.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The Drosophila segment polarity gene lin encodes a protein, lines, which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. [provided by RefSeq, Sep 2010]
Additional Information
Gene ID 55180
Other Names Protein Lines homolog 1 {ECO:0000312|HGNC:HGNC:30922}, Wnt-signaling molecule Lines homolog 1, LINS1 (HGNC:30922)
Target/Specificity Expresssed in adult testis, prostate, prostate, spleen, thymus, skeletal muscle, fetal kidney and brain.
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name LINS1 (HGNC:30922)
Tissue Location Expressed in adult testis, prostate, prostate, spleen, thymus, skeletal muscle, fetal kidney and brain
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

The Drosophila segment polarity gene lin encodes a protein, lines, which plays important roles in development of the epidermis and hindgut. This gene encodes a protein containing a lines-like domain. This gene is located on chromosome 15 and clustered with the gene encoding ankyrin repeat and SOCS box-containing protein 7. [provided by RefSeq, Sep 2010]

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