ZBTB9/ZNF919 Rabbit pAb

Sample:
Du145(Human) Cell Lysate at 40 ug
Lovo(Human) Cell Lysate at 40 ug
Primary: Anti-ZBTB9/ZNF919 (AP57092) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZBTB9/ZNF919) Polyclonal Antibody, Unconjugated (AP57092) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q96C00
Reactivity	Rat, Human, Mouse
Predicted	Pig, Rabbit, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	50602 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human ZBTB9/ZNF919
Epitope Specificity	331-430/473
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	ZBTB9 is a 473 amino acid member of the Krüppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZBTB9 contains a BTB domain, also known as a POZ domain, which inhibits DNA binding and mediates homotypic and heterotypic dimerization. Characteristics of the BTB domain suggest that ZBTB43 functions as a transcription regulator.

Additional Information
Gene ID	221504
Other Names	Zinc finger and BTB domain-containing protein 9, ZBTB9
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.