NAP1L4 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NAP1L4) Polyclonal Antibody, Unconjugated (AP57353) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q99733
Reactivity	Human
Predicted	Rat, Pig, Dog, Mouse, Horse, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	42823 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human NAP1L4
Epitope Specificity	2-100/375
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. [provided by RefSeq, Jul 2008]

Additional Information
Gene ID	4676
Other Names	Nucleosome assembly protein 1-like 4, Histone chaperone NAP1L4, Nucleosome assembly protein 2, NAP-2, NAP1L4 (HGNC:7640)
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	NAP1L4 (HGNC:7640)
Function	Acts as a histone chaperone in nucleosome assembly.
Cellular Location	Nucleus. Chromosome {ECO:0000250\|UniProtKB:Q78ZA7} Cytoplasm Note=Present in the cytoplasm and excluded from the nucleus during G0/G1 phase, then relocates to the nucleus by the time cells are in S phase (PubMed:9325046). Phosphorylated form localizes in the cytoplasm during the G0/G1 transition, whereas dephosphorylation leads to relocalization into the nucleus at the G1/S-boundary (PubMed:10764593)
Tissue Location	Ubiquitous. Biallelically expressed in fetal and adult tissues. Highest levels in testis.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.