癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NDST4 Rabbit pAb
NDST4 Rabbit pAb
- 产品详情
- 实验流程
- 背景知识
Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q9H3R1 |
| Reactivity | Mouse |
| Predicted | Human, Rat, Chicken, Dog, Pig, Horse, Rabbit, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 100716 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human NDST4 |
| Epitope Specificity | 101-200/872 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Golgi apparatus membrane; Single-pass type II membrane protein |
| SIMILARITY | Belongs to the sulfotransferase 1 family. NDST subfamily. |
| SUBUNIT | Monomer |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | The N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin initiate a set of biochemical reactions, which lead to the synthesis of oligosaccharide sequences that have specific ligand binding properties (1). These reactions are catalyzed by the monomeric enzymes GlcNAc Ndeacetylase/N-sulfotransferases (NDSTs), which have two catalytic activities (1). Multiple NDST isozymes have been identified, each having unique tissue distribution and enzymatic properties (2). Phylogenetic data suggests that NDST1-4 evolved from a common ancestral gene, which diverged to give rise to two subtypes, NDST1/2 and NDST3/4 (2). NDST1, which maps to human chromosome 5q32-q33.1, shares the most homology with NDST2, which maps to human chromosome 10q22 (3,4). The least conserved amino acids between these two enzymes are found in the N-terminus/putative transmembrane regions (3). The human NDST3 and NDST4 genes are closely linked on chromosome 4, mapping to chromosome 4q25-26 and 4q26-27, respectively (2,5,6). RT-PCR analysis of various mouse tissues reveals a restricted pattern of NDST3 and NDST4 mRNA expression when compared with that of NDST1 and NDST2, which are abundantly and ubiquitously expressed (2). |
| Gene ID | 64579 |
|---|---|
| Other Names | Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4, 2.8.2.8, Glucosaminyl N-deacetylase/N-sulfotransferase 4, NDST-4, N-heparan sulfate sulfotransferase 4, N-HSST 4, Heparan sulfate N-deacetylase 4, 3.-.-.-, Heparan sulfate N-sulfotransferase 4, 2.8.2.-, NDST4, HSST4 |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| Name | NDST4 |
|---|---|
| Synonyms | HSST4 |
| Function | Essential bifunctional enzyme that catalyzes both the N- deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity (By similarity). |
| Cellular Location | Golgi apparatus membrane; Single- pass type II membrane protein |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin initiate a set of biochemical reactions, which lead to the synthesis of oligosaccharide sequences that have specific ligand binding properties (1). These reactions are catalyzed by the monomeric enzymes GlcNAc Ndeacetylase/N-sulfotransferases (NDSTs), which have two catalytic activities (1). Multiple NDST isozymes have been identified, each having unique tissue distribution and enzymatic properties (2). Phylogenetic data suggests that NDST1-4 evolved from a common ancestral gene, which diverged to give rise to two subtypes, NDST1/2 and NDST3/4 (2). NDST1, which maps to human chromosome 5q32-q33.1, shares the most homology with NDST2, which maps to human chromosome 10q22 (3,4). The least conserved amino acids between these two enzymes are found in the N-terminus/putative transmembrane regions (3). The human NDST3 and NDST4 genes are closely linked on chromosome 4, mapping to chromosome 4q25-26 and 4q26-27, respectively (2,5,6). RT-PCR analysis of various mouse tissues reveals a restricted pattern of NDST3 and NDST4 mRNA expression when compared with that of NDST1 and NDST2, which are abundantly and ubiquitously expressed (2).
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