癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Punt Rabbit pAb
Punt Rabbit pAb
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Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q24468 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 58648 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from Fruit Fly Punt |
| Epitope Specificity | 421-516/516 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Fruit fly (Drosophila melanogaster) ovaries contains two set of germline stem cells surrounded by a group of highly differentiated somatic cells that express genes for two phenotypes (hedgehog and wingless). The TGF beta super family member, decpentaplegic (dpp) or its homologue BMP2/4 is specifically required for maintenance and promotes its cell division in the female germline. The Signaling by TGF beta related factors requires ligand induced association between type I and type II transmembrane receptors that have endogenous serine/threonine kinases activity. In Drosophila, the type I receptor is encoded by the thick veins (tkv) gene and the type II receptor is encoded by the punt (put) gene. These receptors mediate signaling by decapentaplegic (dpp), a member of the bone morphogenetic protein (BMP) subgroup of TGF beta type factors. Over expression or mutation in dpp suppress germline stem cell differentiation. The Drosophila punt gene encodes a type II serine/threonine kinase TGF beta/Dpp (Decapentaplegic) receptor. Dpp actions are mediated by its receptor Punt and Saxophone. There are 5 down stream component in the dpp signaling cascade required to block the development of various organelles including salivary glands. These are Mothers against dpp (Mad), Medea (Med) and Schnurri (Shn). Punt signaling is also responsible for calcium gradient formation during D. melanogaster development. Punt gene encodes for a homolog of vertebrate type II receptor and Punt, like thick veins (Tkv) is essential for in vivo dpp dependent patterning process. No Dpp dependent signal processing is apparent in the absence of Punt ot Tkv suggesting that both receptors acts in concert to transduce Dpp signaling. |
| Gene ID | 41772 |
|---|---|
| Other Names | Receptor protein serine/threonine kinase put {ECO:0000255|RuleBase:RU361271}, 2.7.11.30 {ECO:0000255|RuleBase:RU361271}, Activin type II receptor, Protein punt {ECO:0000303|PubMed:7697720, ECO:0000312|FlyBase:FBgn0003169}, put {ECO:0000312|FlyBase:FBgn0003169} |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | put {ECO:0000312|FlyBase:FBgn0003169} |
|---|---|
| Function | Type II transmembrane receptor serine/threonine kinase involved in activin-like TGF-beta signaling and dpp/BMP-like signaling pathways (PubMed:19766717, PubMed:22563507, PubMed:7697719, PubMed:7697720, PubMed:8289834, PubMed:8415726). Forms a complex with type I transmembrane receptor serine/threonine kinases, and upon activation by signaling ligands induces the type I receptor to phosphorylate downstream receptor-regulated SMAD (R-SMAD) transcription factors, inducing their nuclear localization and resulting in regulation of ligand-responsive genes (PubMed:17119022, PubMed:18820452, PubMed:7697720, PubMed:8289834). Receptor for the activin-like TGF-beta ligands daw and Actbeta/activin when in complex with the type I receptor babo (PubMed:10320478, PubMed:17119022, PubMed:18820452, PubMed:8289834). Activation by daw requires babo isoform C (PubMed:19766717). Receptor for the BMP-like ligand dpp when associated with the type I receptors tkv or sax (PubMed:7697719, PubMed:7697720). Activation by activin-like TGF-beta ligands, including daw and Actbeta/activin, results in phosphorylation of the R-SMAD Smox/SMAD2 (PubMed:10320478, PubMed:17119022, PubMed:18820452, PubMed:19766717, PubMed:8289834). Activation by BMP-like ligands, including gbb and dpp, results in phosphorylation of the R-SMAD Mad (PubMed:18820452, PubMed:19766717). Involved in dpp-signaling-dependent dorsoventral patterning of the epidermis during embryogenesis (PubMed:7697719, PubMed:7697720, PubMed:9504926). Involved in axonal and dendritic pruning and neural remodeling of mushroom body gamma neurons during metamorphosis, probably through regulating expression of the ecdysteroid receptor EcR; functions redundantly with wit (PubMed:12581521). Involved in dpp/BMP-like signaling during development of the embryonic gut tracheal system (PubMed:7697719, PubMed:7697720). Required for dpp/BMP-like signaling involved in imaginal disc development (PubMed:9504926). Involved in differentiation of dorsal cluster and ellipsoid body adult-specific neurons prior to metamorphosis (PubMed:16437159). Required in motoneurons for axon guidance or outgrowth of ISNb and SNa axons (PubMed:17119022). Required in somatic cyst cells for regulation of germ cell proliferation in testis (PubMed:9334286). Involved in adult wing development and wing- vein formation (PubMed:7697720). |
| Cellular Location | Membrane {ECO:0000255|RuleBase:RU361271}; Single- pass type I membrane protein {ECO:0000255|RuleBase:RU361271} Basolateral cell membrane; Single-pass type I membrane protein {ECO:0000255|RuleBase:RU361271}. Apical cell membrane; Single-pass type I membrane protein {ECO:0000255|RuleBase:RU361271}. Note=Localization to different regions of the cell membrane in polarized epithelial cells can differ by tissue and is distinct from wit/wishful thinking, the other type II receptor involved in BMP-like signaling (PubMed:35594316). Highly restricted to the basolateral cell membrane in wing disc epithelial cells; basolateral localization is dependent on the BLT motif and on the AP-1 adapter protein complex (PubMed:35594316). In larval salivary epithelial cells localization is restricted to the basolateral membrane and excluded from cell junctions (PubMed:35594316). Localizes to both the basolateral and apical membrane in follicle cells of the egg chamber (PubMed:35594316). |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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