R3HCC1 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (R3HCC1) Polyclonal Antibody, Unconjugated (AP57630) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q9Y3T6
Reactivity	Rat
Predicted	Cat, Dog, Human, Mouse, Chicken, Horse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	49092 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human R3HCC1
Epitope Specificity	121-220/380
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	R3HCC1 (R3H Domain And Coiled-Coil Containing 1) is a Protein Coding gene. GO annotations related to this gene include nucleic acid binding and nucleotide binding. An important paralog of this gene is R3HCC1L.

Additional Information
Gene ID	203069
Other Names	R3H and coiled-coil domain-containing protein 1 {ECO:0000312\|HGNC:HGNC:27329}, R3HCC1 (HGNC:27329)
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.