SGT1 protein Rabbit pAb

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, E
Primary Accession	O95905
Predicted	Human, Mouse, Rat, Pig, Rhesus
Host	Rabbit
Clonality	Polyclonal
Calculated MW	72758 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SGT1 protein
Epitope Specificity	1-100/644
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasmic and Nuclear
SIMILARITY	Belongs to the SGT1 family.
SUBUNIT	Interacts with TP53 and MDM2.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Gene ID	11319
Other Names	Protein ecdysoneless homolog, Human suppressor of GCR two, hSGT1, ECD
Target/Specificity	Highly expressed in muscle and heart.
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	ECD
Function	Regulator of p53/TP53 stability and function. Inhibits MDM2- mediated degradation of p53/TP53 possibly by cooperating in part with TXNIP (PubMed:16849563, PubMed:23880345). May be involved transcriptional regulation. In vitro has intrinsic transactivation activity enhanced by EP300. May be a transcriptional activator required for the expression of glycolytic genes (PubMed:19919181, PubMed:9928932). Involved in regulation of cell cycle progression. Proposed to disrupt Rb-E2F binding leading to transcriptional activation of E2F proteins (PubMed:19640839). The cell cycle -regulating function may depend on its RUVBL1-mediated association with the R2TP complex (PubMed:26711270). May play a role in regulation of pre-mRNA splicing (PubMed:24722212). Participates together with DDX39A in mRNA nuclear export (PubMed:33941617).
Cellular Location	Cytoplasm. Nucleus Note=Predominantly is located in the cytoplasm
Tissue Location	Highly expressed in muscle and heart. Over- expressed in pancreatic and breast cancers