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PARP14 Rabbit pAb
PARP14 Rabbit pAb
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Application 
| IHC-P, IHC-F, IF |
|---|---|
| Primary Accession | Q460N5 |
| Reactivity | Rat |
| Predicted | Pig, Dog, Human, Mouse, Rabbit, Zebrafish, Horse, Sheep |
| Host | Rabbit |
| Clonality | Polyclonal |
| Calculated MW | 202800 Da |
| Physical State | Liquid |
| Immunogen | KLH conjugated synthetic peptide derived from human PARP14 |
| Epitope Specificity | 1651-1750/1801 |
| Isotype | IgG |
| Purity | affinity purified by Protein A |
| Buffer | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| SUBCELLULAR LOCATION | Nucleus. Cytoplasm. In steady state cells the protein is mostly nuclear. A minor proportion is detected in the cytoplasm. |
| Important Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| Background Descriptions | Poly(ADP-ribosyl)ation is an immediate DNA damage-dependent posttranslational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP14 belongs to the superfamily of enzymes that perform this modification (Ame et al., 2004 [PubMed 15273990]).[supplied by OMIM, Mar 2008] |
| Gene ID | 54625 |
|---|---|
| Other Names | Protein mono-ADP-ribosyltransferase PARP14, 2.4.2.-, ADP-ribosyltransferase diphtheria toxin-like 8, ARTD8, B aggressive lymphoma protein 2, Poly [ADP-ribose] polymerase 14, PARP-14, PARP14 {ECO:0000303|PubMed:20106667, ECO:0000312|HGNC:HGNC:29232} |
| Dilution | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500 |
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | PARP14 {ECO:0000303|PubMed:20106667, ECO:0000312|HGNC:HGNC:29232} |
|---|---|
| Function | ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed:16061477, PubMed:18851833, PubMed:25043379, PubMed:27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed:27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed:29858569). Mono-ADP- ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed:27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed:27796300). Mono-ADP-ribosylates PARP9 (PubMed:27796300). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q2EMV9}. Cytoplasm. Note=In steady state splenocytes the protein is mostly nuclear (By similarity). A minor proportion is detected in the cytoplasm (By similarity). In macrophages, mainly localizes to the cytoplasm (PubMed:27796300) {ECO:0000250|UniProtKB:Q2EMV9, ECO:0000269|PubMed:27796300} |
| Tissue Location | Expressed in macrophages. |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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