TMEM109 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample:
U251(Human) Cell Lysate at 40 ug
Panc-1(Human) Cell Lysate at 40 ug
Primary: Anti-TMEM109 (bs-10196R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 23 kD
Observed band size: 23 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM109) Polyclonal Antibody, Unconjugated (AP57727) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM109) Polyclonal Antibody, Unconjugated (AP57727) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q9BVC6
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	26210 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human TMEM109
Epitope Specificity	151-243/243
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus outer membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Additional Information
Gene ID	79073
Other Names	Transmembrane protein 109, Mitsugumin-23, Mg23, TMEM109
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	TMEM109 (HGNC:28771)
Function	Functions as a voltage-gated monoatomic cation channel permeable to both potassium and calcium (By similarity). Plays a role in the cellular response to DNA damage (PubMed:23542032).
Cellular Location	Nucleus outer membrane {ECO:0000250\|UniProtKB:O77751}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:O77751}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:O77751}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:O77751}. Sarcoplasmic reticulum membrane {ECO:0000250\|UniProtKB:O77751}; Multi-pass membrane protein {ECO:0000250\|UniProtKB:O77751}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.