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COUP-TF1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - COUP-TF1 Polyclonal Antibody AP57796
    Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (COUP-TF1) Polyclonal Antibody, Unconjugated (AP57796) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF, ICC
Primary Accession P10589
Reactivity Rat, Pig, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 46156 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human COUP-TF1
Epitope Specificity 21-120/423
Isotype IgG
Purity affinity purified by Protein A
Buffer Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBCELLULAR LOCATION Nucleus.
SIMILARITY Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain.
SUBUNIT Binds DNA as dimer; homodimer and probable heterodimer with NR2F6. Interacts with COPS2.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions COUP transcription factors have been cloned in several species and identified as orphan members of the steroid/thyroid hormone receptor superfamily. COUP-TFI (also designated COUP or EAR-3) and ARP-1 (also designated COUP-TFII) exhibit highly regulated and overlapping expression in most tissues. COUP-TFs are highly expressed in the developing and central nervous system, suggesting that these factors may be important in neural development and differentiation. COUP-TFs can compete for binding to response elements which are common to other members of this family, including RAR, RXR, PPAR, HNF-4, VDR and TR. They have been shown to act as negative regulators as well as initiators of transcription.
Additional Information
Gene ID 7025
Other Names COUP transcription factor 1, COUP-TF1, COUP transcription factor I, COUP-TF I, Nuclear receptor subfamily 2 group F member 1, V-erbA-related protein 3, EAR-3, NR2F1, EAR3, ERBAL3, TFCOUP1
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name NR2F1
Synonyms EAR3, ERBAL3, TFCOUP1
Function Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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