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SIGLEC1 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Sialoadhesin) Polyclonal Antibody, Unconjugated (AP57798) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q62230
Reactivity	Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	182979 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from mouse Sialoadhesin/CD169
Epitope Specificity	1301-1400/1709
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008].

Additional Information
Gene ID	20612
Other Names	Sialoadhesin, Sheep erythrocyte receptor, SER, Sialic acid-binding Ig-like lectin 1, Siglec-1, CD169, Siglec1, Sa, Sn
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Siglec1
Synonyms	Sa, Sn
Function	Macrophage-restricted adhesion molecule that mediates sialic- acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Plays a crucial role in limiting bacterial dissemination by engaging sialylated bacteria to promote effective phagocytosis and antigen presentation for the adaptive immune response (PubMed:24788876). Mediates the uptake of various enveloped viruses via sialic acid recognition and subsequently induces the formation of intracellular compartments filled with virions (VCCs) (PubMed:26370074). In turn, enhances macrophage-to-T-cell transmission of several viruses including murine leukemia virus (PubMed:26370074). Acts as an endocytic receptor mediating clathrin dependent endocytosis. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. Plays a role in the inhibition of antiviral innate immune by promoting TBK1 degradation via TYROBP and TRIM27- mediated ubiquitination (By similarity).
Cellular Location	[Isoform 1]: Cell membrane; Single-pass type I membrane protein [Isoform 3]: Secreted.
Tissue Location	Detected in lymph node in the subcapsular sinus, interfollicular regions, and T and B-cell boundary (at protein level) Expressed by macrophages in various tissues (PubMed:26370074). Highest expression in spleen and lymph node with lower amounts in lung, liver, bone marrow, heart and skin. No expression in thymus, kidney, brain or small intestine.