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POU2F3 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - POU2F3 Polyclonal Antibody AP57840
    Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POU2F3) Polyclonal Antibody, Unconjugated (AP57840) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
  • 1 - POU2F3 Polyclonal Antibody AP57840
    Sample:
    Liver (Mouse) Lysate at 40 ug
    Primary: Anti- POU2F3 (AP57840) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 47 kD
    Observed band size: 48 kD
  • 1 - POU2F3 Polyclonal Antibody AP57840
    Sample:
    Testis (Mouse) Lysate at 40 ug
    Primary: Anti- POU2F3 (AP57840) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 47 kD
    Observed band size: 48 kD
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9UKI9
Reactivity Rat, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 47432 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human POU2F3
Epitope Specificity 1-100/436
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus.
SIMILARITY Belongs to the POU transcription factor family. Class-2 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions This gene encodes a member of the POU domain family of transcription factors. POU domain transcription factors bind to a specific octamer DNA motif and regulate cell type-specific differentiation pathways. The encoded protein is primarily expressed in the epidermis, and plays a critical role in keratinocyte proliferation and differentiation. The encoded protein is also a candidate tumor suppressor protein, and aberrant promoter methylation of this gene may play a role in cervical cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Additional Information
Gene ID 25833
Other Names POU domain, class 2, transcription factor 3, Octamer-binding protein 11, Oct-11, Octamer-binding transcription factor 11, OTF-11, Transcription factor PLA-1, Transcription factor Skn-1, POU2F3, OTF11, PLA1
Target/Specificity Specifically expressed in epidermis and cultured keratinocytes.
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name POU2F3 (HGNC:19864)
Synonyms OTF11, PLA1
Function Transcription factor that binds to the octamer motif (5'- ATTTGCAT-3') and regulates cell type-specific differentiation pathways. Involved in the regulation of keratinocytes differentiation (PubMed:11329378). The POU2F3-POU2AF2/POU2AF3 complex drives the expression of tuft-cell-specific genes, a rare chemosensory cells that coordinate immune and neural functions within mucosal epithelial tissues (PubMed:35576971).
Cellular Location Nucleus {ECO:0000250|UniProtKB:P31362}.
Tissue Location Specifically expressed in epidermis and cultured keratinocytes.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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