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OPA3 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - OPA3 Polyclonal Antibody AP57867
    Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OPA3) Polyclonal Antibody, Unconjugated (AP57867) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF, ICC
Primary Accession Q9H6K4
Reactivity Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 19996 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human OPA3
Epitope Specificity 1-100/179
Isotype IgG
Purity affinity purified by Protein A
Buffer Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBCELLULAR LOCATION Mitochondrial.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions The mouse ortholog of this protein co-purifies with the mitochondrial inner membrane. Mutations in this gene have been shown to result in 3-methylglutaconic aciduria type III and autosomal dominant optic atrophy and cataract. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]
Additional Information
Gene ID 80207
Other Names Optic atrophy 3 protein, OPA3
Dilution IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name OPA3
Function May play some role in mitochondrial processes.
Cellular Location Mitochondrion.
Tissue Location Ubiquitous. Most prominent expression in skeletal muscle and kidney.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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