RALGPS2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (RALGPS2) Polyclonal Antibody, Unconjugated (AP57915) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, ICC
Primary Accession	Q86X27
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	65167 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human RALGPS2
Epitope Specificity	481-583/583
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBCELLULAR LOCATION	Cell Membrane and Cytoplasmic. Associates with membranes through the PH domain.
SUBUNIT	Interacts with the SH3 domains of GRB2 and PLCG1. Interacts with RALA.
DISEASE	RALGPS2 is a potential candidate gene for susceptibility to Alzheimer disease linked to 1q24.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	RalGPS2 (Ral GEF with PH domain and SH3 binding motif 2) is a guanine nucleotide exchange factor for RalA GTPase. It is expressed in testis and brain.

Additional Information
Gene ID	55103
Other Names	Ras-specific guanine nucleotide-releasing factor RalGPS2, Ral GEF with PH domain and SH3-binding motif 2, RalA exchange factor RalGPS2, RALGPS2
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	RALGPS2
Function	Guanine nucleotide exchange factor for the small GTPase RALA. May be involved in cytoskeletal organization. May also be involved in the stimulation of transcription in a Ras-independent fashion (By similarity).
Cellular Location	Cytoplasm. Cell membrane. Note=Associates with membranes through the PH domain

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.