TNFSF13B Rabbit pAb

Blank control(blue). : Jurkat cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-BAFF antibody(AP58024), Dilution: 3 µg in 100 µL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Sample:
Lane 1: Recombinant human TNFSF13B protein, N-His(AP58024)
Primary: Anti-TNFSF13B (AP58024) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kDa
Observed band size: 52 kDa
Measured by its binding ability in a indirect ELISA. Immobilized TNFSF13B Peptides(Cat. AP58024) at 2 µg/mL (100 µL/well) can bind Rabbit Anti-TNFSF13B Antibody, the EC50 is 797.5 ng/mL.
Blank control(blue). : Jurkat cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-BAFF antibody(AP58024), Dilution: 3 µg in 100 µL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	Q9WU72
Reactivity	Human
Predicted	Rat, Pig, Mouse, Rabbit
Host	Rabbit
Clonality	Polyclonal
Calculated MW	34192 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from mouse TNFSF13B
Epitope Specificity	221-309/309
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Secreted and Cell membrane.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptors TNFRSF13B/TACI, TNFRSF17/BCMA, and TNFRSF13C/BAFFR. This cytokine is expressed in B cell lineage cells, and acts as a potent B cell activator. It has been also shown to play an important role in the proliferation and differentiation of B cells. [provided by RefSeq].

Additional Information
Gene ID	24099
Other Names	Tumor necrosis factor ligand superfamily member 13B, B-cell-activating factor, BAFF, CD257, Tumor necrosis factor ligand superfamily member 13b, membrane form, Tumor necrosis factor ligand superfamily member 13b, soluble form, Tnfsf13b, Baff
Dilution	WB=1:500-2000,Flow-Cyt=3 µg/Test,ELISA=1:5000-10000
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Tnfsf13b
Synonyms	Baff
Function	Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T- cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B- cells and the B-cell response.
Cellular Location	Cell membrane; Single-pass type II membrane protein
Tissue Location	Isoform 2 is expressed in many myeloid cell lines.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.