ID2 Rabbit pAb

Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-ID2 antibody (AP58073)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ID2) Polyclonal Antibody, Unconjugated (AP58073) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q02363
Reactivity	Rat, Human
Predicted	Pig, Mouse, Rabbit, Chicken, Dog, Horse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	14917 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human GIG8/ID2
Epitope Specificity	31-134/134
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm. Nucleus.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	The protein encoded by this gene belongs to the inhibitor of DNA binding (ID) family, and may play a role in negatively regulating cell differentiation. Members of the ID family are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. They inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner, by suppressing their heterodimerization partners through the HLH domains.

Additional Information
Gene ID	3398
Other Names	DNA-binding protein inhibitor ID-2, Class B basic helix-loop-helix protein 26, bHLHb26, Inhibitor of DNA binding 2, Inhibitor of differentiation 2, ID2, BHLHB26
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=2ug/Test
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	ID2
Synonyms	BHLHB26
Function	Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Restricts the CLOCK and BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
Cellular Location	Cytoplasm {ECO:0000250\|UniProtKB:P41136}. Nucleus {ECO:0000250\|UniProtKB:P41136}
Tissue Location	Highly expressed in early fetal tissues, including those of the central nervous system

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.