ITPR2 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ITPR2 Polyclonal Antibody, Unconjugated(AP58137) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF, ICC, E
Primary Accession	Q14571
Reactivity	Rat, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	308064 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human ITPR2
Epitope Specificity	2351-2450/2701
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Endoplasmic reticulum membrane; Multi-pass membrane protein.
SIMILARITY	Belongs to the InsP3 receptor family. Contains 5 MIR domains.
SUBUNIT	Homotetramer. Interacts with CABP1.
Post-translational modifications	Phosphorylation by cAMP-dependent PKA on Ser-937 increases calcium release.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium.

Additional Information
Gene ID	3709
Other Names	Inositol 1, 4, 5-trisphosphate receptor type 2, IP3 receptor isoform 2, IP3R 2, InsP3R2, Type 2 inositol 1, 4, 5-trisphosphate receptor, Type 2 InsP3 receptor, ITPR2
Target/Specificity	Isoform Short is found in skeletal muscle and heart.
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	ITPR2 (HGNC:6181)
Function	Inositol 1,4,5-trisphosphate-gated calcium channel that upon inositol 1,4,5-trisphosphate binding transports calcium from the endoplasmic reticulum lumen to cytoplasm. Exists in two states; a long- lived closed state where the channel is essentially 'parked' with only very rare visits to an open state and that ligands facilitate the transition from the 'parked' state into a 'drive' mode represented by periods of bursting activity (By similarity).
Cellular Location	Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:Q9Z329}; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250\|UniProtKB:Q8WN96}; Multi-pass membrane protein. Note=Forms clusters on endoplasmic reticulum membrane upon inositol 1,4,5-trisphosphate binding {ECO:0000250\|UniProtKB:Q9Z329}
Tissue Location	[Isoform Short]: Found in skeletal muscle and heart.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.