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ATP1A2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ATP1A2 Antibody (Center) AP5828c
    All lanes: Anti-ATP1A2 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Mouse heart lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 112 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - ATP1A2 Antibody (Center) AP5828c
    Confocal immunofluorescent analysis of ATP1A2 Antibody (Center)(Cat. #AP5828c) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
  • 1 - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) western blot analysis in MCF-7 cell line lysates (15ug/lane).This demonstrates the ATP1A2 antibody detected ATP1A2 protein (arrow).
  • 14 - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP1A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - ATP1A2 Antibody (Center) AP5828c
    ATP1A2 Antibody (Center) (Cat. #AP5828c) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, FC, E
Primary Accession P50993
Other Accession P06686, D2WKD8, Q6PIE5, A2VDL6, NP_000693.1
Reactivity Human, Mouse
Predicted Mouse, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 112265 Da
Antigen Region 451-479 aa
Additional Information
Gene ID 477
Other Names Sodium/potassium-transporting ATPase subunit alpha-2, Na(+)/K(+) ATPase alpha-2 subunit, Sodium pump subunit alpha-2, ATP1A2, KIAA0778
Target/Specificity This ATP1A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human ATP1A2.
Dilution WB~~1:1000
IHC-P~~1:100~500
IF~~1:10~50
FC~~1:10~50
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATP1A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ATP1A2
Synonyms KIAA0778
Function This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium, providing the energy for active transport of various nutrients.
Cellular Location Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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