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DBF4B Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample: 293T Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (AP58303) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
Sample: Hela Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (AP58303) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD
Sample: U2os Cell (Human) Lysate at 30 ug
Primary: Anti-DBF4B (AP58303) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 18kD

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	Q8NFT6
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	67243 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human DBF4B/ASKL1
Epitope Specificity	41-140/615
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction.
SIMILARITY	Contains 1 BRCT domain. Contains 1 DBF4-type zinc finger.
SUBUNIT	Forms a complex with CDC7. Note that CDC7 forms distinct complex either with DBF4/DBF4A or DBF4B. Such complexes are stable upon replication stress.
Post-translational modifications	Phosphorylated.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	DBF4B is a regulatory subunit for CDC7, a serine-threonine kinase which links cell cycle regulation to genome duplication. The complex CDC7-DBF4B selectively phosphorylates the MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. DBF4B localizes to the nucleus and its expression is cell cycle-regulated.

Additional Information
Gene ID	80174
Other Names	Protein DBF4 homolog B, Activator of S phase kinase-like protein 1, ASK-like protein 1, Chiffon homolog B, Dbf4-related factor 1, DBF4B, ASKL1, DRF1
Target/Specificity	Widely expressed. Highly expressed in testis.
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	DBF4B
Synonyms	ASKL1, DRF1
Function	Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle.
Cellular Location	Nucleus. Note=Predominantly found in soluble fraction but not in the chromatin-bound fraction
Tissue Location	Widely expressed. Highly expressed in testis.

Research Areas