OVCA2 Rabbit pAb

Tissue/cell: Rat ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-OVCA2 Polyclonal Antibody, Unconjugated(AP58414) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q8WZ82
Reactivity	Rat
Predicted	Pig, Dog, Human, Mouse, Rabbit, Horse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	24418 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human OVCA2
Epitope Specificity	11-100/227
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Ubiquitously expressed protein which belongs to the UPF0483 (OVCA2) family. Encoded in an intron of the gene DPH1/OVCA1 (same strand).

Additional Information
Gene ID	124641
Other Names	Esterase OVCA2, 3.1.1.1, OVCA2 serine hydrolase domain-containing protein, Ovarian cancer-associated gene 2 protein, OVCA2 (HGNC:24203)
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	OVCA2 (HGNC:24203)
Function	Exhibits ester hydrolase activity with a strong preference for long-chain alkyl ester substrates and high selectivity against a variety of short, branched, and substituted esters. Is able to hydrolyze ester bonds within a wide range of p-nitrophenyl derivatives (C2-C14) in vitro, with a strong preference toward substrates of >8 carbons.
Tissue Location	Ubiquitously expressed.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.