OCIAD1 Rabbit pAb

25 ug total protein per lane of various lysates (see on figure) probed with OCIAD1 polyclonal antibody, unconjugated (AP58567) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OCIAD1) Polyclonal Antibody, Unconjugated (AP58567) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF
Primary Accession	Q9NX40
Reactivity	Rat, Mouse
Predicted	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	27626 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human OCIAD1
Epitope Specificity	76-170/245
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Endosome.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Tissue specificity; Isoform 1 is highly expressed in many tissues, including testis, brain, placenta, ovary, prostate and mammary gland. Isoform 2 expression is restricted to the central nervous system including brain, cerebellum and spinal cord.

Additional Information
Gene ID	54940
Other Names	OCIA domain-containing protein 1, Ovarian cancer immunoreactive antigen domain containing 1, Ovarian carcinoma immunoreactive antigen, OCIAD1 (HGNC:16074)
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	OCIAD1 (HGNC:16074)
Function	Maintains stem cell potency (By similarity). Increases STAT3 phosphorylation and controls ERK phosphorylation (By similarity). May act as a scaffold, increasing STAT3 recruitment onto endosomes (By similarity). Involved in integrin-mediated cancer cell adhesion and colony formation in ovarian cancer (PubMed:20515946).
Cellular Location	Endosome {ECO:0000250\|UniProtKB:Q9CRD0}.
Tissue Location	Isoform 1 is highly expressed in many tissues, including testis, brain, placenta, ovary, prostate and mammary gland Isoform 2 expression is restricted to the central nervous system including brain, cerebellum and spinal cord

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.