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PARP4 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - PARP4 Polyclonal Antibody AP58599
    Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP4) Polyclonal Antibody, Unconjugated (AP58599) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PARP4) Polyclonal Antibody, Unconjugated (AP58599) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, IHC-F, IF, E
Primary Accession Q9UKK3
Reactivity Rat, Pig
Host Rabbit
Clonality Polyclonal
Calculated MW 192595 Da
Additional Information
Gene ID 143
Other Names Protein mono-ADP-ribosyltransferase PARP4, 2.4.2.-, 193 kDa vault protein, ADP-ribosyltransferase diphtheria toxin-like 4, ARTD4, PARP-related/IalphaI-related H5/proline-rich, PH5P, Poly [ADP-ribose] polymerase 4, PARP-4, Vault poly(ADP-ribose) polymerase, VPARP, PARP4 {ECO:0000303|PubMed:20106667, ECO:0000312|HGNC:HGNC:271}
Dilution IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name PARP4 {ECO:0000303|PubMed:20106667, ECO:0000312|HGNC:HGNC:271}
Function Mono-ADP-ribosyltransferase that mediates mono-ADP- ribosylation of target proteins.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Also found in the nucleus, associated with mitotic spindles.
Tissue Location Widely expressed; the highest levels are in the kidney; also detected in heart, placenta, lung, liver, skeletal muscle, spleen, leukocytes and pancreas.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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