VAMP4 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

VAMP4 Antibody (Center) (Cat. #AP5861c) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VAMP4 antibody detected the VAMP4 protein (arrow).
VAMP4 Antibody (Center) (Cat. #AP5861c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the VAMP4 antibody detected the VAMP4 protein (arrow).
VAMP4 antibody (Center) (Cat. #AP5861c) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the VAMP4 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	O75379
Other Accession	O70480, Q32L97, NP_001172056.1, NP_003753.2
Reactivity	Human, Mouse
Predicted	Bovine
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	16397 Da
Antigen Region	16-45 aa

Additional Information
Gene ID	8674
Other Names	Vesicle-associated membrane protein 4, VAMP-4, VAMP4
Target/Specificity	This VAMP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the Central region of human VAMP4.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	VAMP4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	VAMP4
Function	Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Cellular Location	Golgi apparatus, trans-Golgi network membrane; Single-pass type IV membrane protein Note=Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.