ZNF408 Rabbit pAb

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF408) Polyclonal Antibody, Unconjugated (AP58640) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IHC-F, IF
Primary Accession	Q9H9D4
Reactivity	Mouse
Predicted	Human, Pig, Horse, Rabbit, Sheep
Host	Rabbit
Clonality	Polyclonal
Calculated MW	78439 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human ZNF408
Epitope Specificity	541-640/720
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
SUBCELLULAR LOCATION	Nucleus.
SIMILARITY	Contains 10 C2H2-type zinc fingers.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	ZNF408 (Zinc Finger Protein 408) is a Protein Coding gene. Diseases associated with ZNF408 include exudative vitreoretinopathy 6 and retinitis pigmentosa 72. GO annotations related to this gene include identical protein binding.

Additional Information
Gene ID	79797
Other Names	Zinc finger protein 408, PR domain zinc finger protein 17, ZNF408, PFM14, PRDM17
Dilution	IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	ZNF408
Synonyms	PFM14, PRDM17
Function	May be involved in transcriptional regulation.
Cellular Location	Nucleus
Tissue Location	Highest expression is observed in adult retina; abundantly expressed in the fetal eye (PubMed:23716654). In the retina, it is detected in the outer nuclear layer, especially cone and rod photoreceptor cells, ganglion cell layer and both outer and inner plexiform layers (at protein level) (PubMed:25882705). Expressed in retinal blood vessels (at protein level) (PubMed:25882705)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

ZNF408 (Zinc Finger Protein 408) is a Protein Coding gene. Diseases associated with ZNF408 include exudative vitreoretinopathy 6 and retinitis pigmentosa 72. GO annotations related to this gene include identical protein binding.

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¥ 1,500.00

Cat# AP58640

Size:

50 µl

100 µl

Price:

¥ 1,500.00

¥ 2,600.00

Quantity:

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Availability: 3-5 days

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ZNF408 Rabbit pAb

ZNF408 Rabbit pAb

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