SHOC2 Rabbit pAb

Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Esophagus (Mouse) Lysate at 40 ug
Lane 4: Large intestine (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: Esophagus (Rat) Lysate at 40 ug
Lane 9: Large intestine (Rat) Lysate at 40 ug
Lane 10: A549 (Human) Cell Lysate at 30 ug
Lane 11: U937 (Human) Cell Lysate at 30 ug
Lane 12: HL60 (Human) Cell Lysate at 30 ug
Primary:
Anti- SHOC2 (AP58801) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 60 kD

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	Q9UQ13
Reactivity	Rat, Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Calculated MW	64888 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human SHOC2/Sur8
Epitope Specificity	36-120/582
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION	Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus upon growth factor stimulation.
DISEASE	Defects in SHOC2 are the cause of Noonan syndrome-like disorder with loose anagen hair (NSLH) [MIM:607721]. A syndrome characterized by Noonan dysmorphic features such as macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck, pectus anomalies, in association with pluckable, sparse, thin and slow-growing hair.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	Regulatory subunit of protein phosphatase 1 (PP1c) that acts as a M-Ras/MRAS effector and participates in MAPK pathway activation. Upon M-Ras/MRAS activation, targets PP1c to specifically dephosphorylate the 'Ser-259' inhibitory site of RAF1 kinase and stimulate RAF1 activity at specialized signaling complexes. Involvement in disease:Defects in SHOC2 are the cause of Noonan syndrome-like with loose anagen hair (NSLAH) . NSLAH children display macrocephaly, high forehead, hypertelorism, palpebral ptosis, low-set and posteriorly rotated ears, short and webbed neck and pectus anomalies. Affected subjects also have easily pluckable, sparse, thin and slow-growing hair.

Additional Information
Gene ID	8036
Other Names	Leucine-rich repeat protein SHOC-2, Protein soc-2 homolog, Protein sur-8 homolog, SHOC2, KIAA0862
Dilution	WB=1:500-2000,ELISA=1:5000-10000,ICC/IF=1:50 - 1:100
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	SHOC2
Synonyms	KIAA0862
Function	Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates activation of the MAPK pathway (PubMed:10783161, PubMed:16630891, PubMed:25137548, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). Acts as a scaffolding protein in the SMP complex (PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (PubMed:10783161, PubMed:16630891, PubMed:35768504, PubMed:35830882, PubMed:35831509, PubMed:36175670). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (PubMed:35768504, PubMed:36175670). Acts as an inhibitor of keratinocyte differentiation via interaction with RAS protein complexes that promote ERK activation (PubMed:23524970).
Cellular Location	Cytoplasm. Nucleus. Note=Translocates from cytoplasm to nucleus upon growth factor stimulation.
Tissue Location	Expressed in the intermediate epidermal layers, expression is weaker in the most basal and suprabasal keratinocytes (at protein level).