ANKRD9 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

Sample:
293T(Human) Cell Lysate at 30 ug
Primary: Anti- ANKRD9 (AP58811) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 35 kD
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ANKRD9 Polyclonal Antibody, Unconjugated(AP58811) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IHC-F, IF, E
Primary Accession	Q96BM1
Reactivity	Rat, Pig, Dog, Bovine
Host	Rabbit
Clonality	Polyclonal
Calculated MW	34295 Da
Physical State	Liquid
Immunogen	KLH conjugated synthetic peptide derived from human ANKRD9
Epitope Specificity	21-200/317
Isotype	IgG
Purity	affinity purified by Protein A
Buffer	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SIMILARITY	Contains 3 ANK repeats.
Important Note	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions	ANKRD9 is a 317 amino acid protein that contains three ANK repeats. Encoded by a gene that maps to human chromosome 14q32.31, ANKRD9 is conserved in chimpanzee, dog, cow, mouse, rat and zebrafish. Hepatic mRNA levels of ANKRD9 are repressed by both thyroid hormone (T(3)) and fasting, and re-elevate by feeding after fasting. ANKRD9 mRNA levels also decrease in response to apoptosis. Localizing to cytoplasm, ANKRD9 may be involved in intracellular lipid accumulation and lipid metabolism. ANKRD9 may also function as a molecular chaperone

Additional Information
Gene ID	122416
Other Names	Ankyrin repeat domain-containing protein 9, ANKRD9
Dilution	WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
Format	0.01M TBS(pH7.4) with 1% BSA, 0.09% (W/V) sodium azide and 50% Glyce
Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Protein Information
Name	ANKRD9
Function	Substrate receptor subunit of a cullin-RING superfamily E3 ligase complex (CUL5-based E3 ubiquitin ligase complex) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:30293565). Depending of the metabolic state of the cell, promotes the proteasomal degradation of IMPDH2, the rate-limiting enzyme in GTP biosynthesis or protects IMPDH2 by stabilizing IMPDH2 filaments assembly (PubMed:30293565, PubMed:31337707). Implicated in different cellular processes, like copper homeostasis and cell proliferation (PubMed:24522796, PubMed:30293565).
Cellular Location	Cytoplasmic vesicle. Cytoplasm, cytosol Note=Detected in long filamentous cytosolic structures where it colocalizes with IMPDH2 (PubMed:31337707). Under basal conditions ANKRD9 is mainly in vesicle-like structures, upon nutrient limitation (guanine nucleotides deficiency) ANKRD9 loses its vesicular pattern and assembles with IMPDH2 into rodlike filaments (PubMed:31337707)