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HOXC9 Rabbit pAb

HOXC9 Rabbit pAb

     
  • 1 - HOXC9 Rabbit pAb AP58821
    Sample:
    Lane 1: Muscle (Mouse) Tissue Lysate at 40 ug
    Lane 2: Muscle (Rat) Tissue Lysate at 40 ug
    Lane 3: Ear (Rat) Tissue Lysate at 40 ug
    Primary: Anti-HOXC9 (AP58821) at 1/200 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 29 kD
    Observed band size: 40 kD
  • 14 - HOXC9 Rabbit pAb AP58821
    Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-HOXC9 Polyclonal Antibody, Unconjugated(AP58821) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IHC-F, IF
Primary Accession P31274
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 29248 Da
Physical State Liquid
Immunogen KLH conjugated synthetic peptide derived from human HOXC9
Epitope Specificity 151-220/260
Isotype IgG
Purity affinity purified by Protein A
Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
SUBCELLULAR LOCATION Nucleus.
SIMILARITY Belongs to the Abd-B homeobox family.Contains 1 homeobox DNA-binding domain.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Background Descriptions HOXC9 plays an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem.
Additional Information
Gene ID 3225
Other Names Homeobox protein Hox-C9, Homeobox protein Hox-3B, HOXC9, HOX3B
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name HOXC9
Synonyms HOX3B
Function Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Cellular Location Nucleus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

HOXC9 plays an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem.

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